Isotypes of 78 kD Streptavidin Biotin-binding Proteins (SBP) during Initial Germination of Dark-grown Pea (<i>Pisum sativum</i> L.) Seedlings
Bibliographic record
Abstract
In this study two dimensional page and western blotting have been used to investigate the status of isotypes of 78 kD streptavidin biotin-binding proteins (SBP) in dry seeds, germinated seeds and a combination with its different plant parts including stems, plumules and roots of pea ( Pisum sativum L. var Alaska). Dry seeds contain 21 isotypes, five of them are almost separated with isoelectric point (PI) 5.66, 5.82, 5.88, 5.91 and 6.55, and four of them are partially separated with pI values 5.92, 5.94, 5.96 and 5.98, the remaining forming unseparated isotypes mixture with pI values varied from 5.07 to 6.50. Embryos of forty hours germinated pea seeds showed 9 different biotin isotypes with pI values from 5.76 to 6.64. Stems parts of seventy two hours germinated pea seeds showed highest expressed 11 biotin isotypes with pI values from 5.60 to 6.65, whereas the plumules parts showed 10 isotypes with pI values from 5.78 to 6.46. And the root parts showed 5 separated isotypes with PI values from 5.72 to 6.00 and unseparated isotypes mixture with a pI values ranged from 6.27 to 6.67. These results suggest that the isotypes of 78 kD streptavidin biotin-binding proteins in dormant and germinating pea seeds might play a role in embryo longevity and early seed germination.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".