Ploidy Level and DNA Content of Perennial Ryegrass Germplasm as Determined by Flow Cytometry
Why this work is in the frame
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Bibliographic record
Abstract
Perennial ryegrass ( Lolium perenne L.) is a widely used cool-season turfgrass species. The exact ploidy levels of the worldwide perennial ryegrass accessions in the USDA National Plant Germplasm System (NPGS) are unknown, which could complicate future use and breeding efforts. The objective of this study was to determine the ploidy level and DNA content of the 194 USDA NPGS perennial ryegrass accessions and six commercial cultivars (Brightstar SLT, Catalina II, Divine, Inspire, Manhattan 4, Silver Dollar) using flow cytometry. Among the 200 accessions, 194 diploids and six tetraploids were identified. Three tetraploids originated from Canada with the remaining from Ireland, Japan, and The Netherlands. The average DNA content was 5.60 pg/2C for the diploid and 11.45 pg/2C for the tetraploid. The 2C DNA content was positively correlated ( r = 0.23, P < 0.01) with seedling plant height but not seedling leaf width. This ploidy data provide important information for future marker trait analysis and cultivar improvement.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it