Metabotropic Induction of Persistent Activity in Layers II/III of Anterior Cingulate Cortex
Bibliographic record
Abstract
The anterior cingulate cortex (ACC), a limbic region associated with pain-related working memory and memory acquisition, receives a dense cholinergic innervation. To further understand the role of acetylcholine in ACC, we characterized the firing properties of pyramidal neurons following muscarinic receptor activation. Using whole-cell patch clamp recordings in acute brain slices, we report long-lasting nonsynaptic plateau potentials and persistent firing induced by carbachol (CCh) in pyramidal neurons in layers II/III of rat ACC. CCh responses were abolished by the muscarinic receptor antagonist atropine or by inhibitors of G proteins and phospholipase C. Inhibiting L-type calcium channels with nifedipine, removing extracellular calcium or chelating intracellular calcium with BAPTA also abolished plateau potentials and persistent firing. Blockade of nonselective cation channels with flufenamic acid, 2-aminoethyl diphenylborinate or SKF-96365 suppressed CCh responses and voltage-clamp recordings of CCh-sensitive currents revealed a transient receptor potential canonical-like cationic conductance. The group I metabotropic glutamate receptor (mGluR) agonist (S)-3,5-dihydroxyphenylglycine hydrate induced plateau potentials and persistent firing that were mediated by mGluR5. Our data demonstrate that receptor-operated channels drive calcium-dependent plateau potentials and persistent firing in layers II/III of ACC. Therefore, acetylcholine- and glutamate-evoked persistent activity in ACC may play a mnemonic role by allowing transient storage of information during pain processing.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".