Inclusion body hepatitis as a primary disease in commercial broiler chickens
Bibliographic record
Abstract
Inclusion body hepatitis (IBH) has been occurring as an economically important, emerging disease of broiler chickens in several countries.Historically, IBH has been identified as a secondary disease, often associated with common immunosuppressive diseases.However, few studies have identified IBH as a primary disease with no apparent association with immunosuppressive diseases.The objectives of this study were to develop an animal model of IBH in commercial broilers, to demonstrate vertical transmission of fowl adenoviruses (FAdVs) in broiler breeders and to control IBH in broilers by vaccinating their parents with an inactivated FAdV vaccine.In order to develop an animal model of IBH in commercial broilers, fourteenday old broilers were inoculated intramuscularly with 1x10 4 -1x10 7 CCID 50 of either FAdV x11a-like virus, two strains of FAdV-8a (FAdV-8a strain TR-59 and FAdV-8a strain T8-A) or FAdV-11strain 1047.Four days following FAdV inoculation, 5% -15% mortality was observed and dead birds showed histologic lesions of hemorrhagic necrotizing hepatitis.This animal model reproduced the clinical disease, and pathological lesions of IBH that have been described in commercial broilers.In order to demonstrate vertical transmission of the FAdV, 35-week-old broiler breeders were inoculated with 1x10 6 CCID 50 of either FAdV x11a-like virus, FAdV-8a strain TR-59, FAdV-8a strain T8-A or FAdV-11 strain 1047.Eggs from infected breeders were collected and hatched seven days post-inoculation.Clinical signs or mortality were not observed in parents; however broiler progeny derived from broiler breeders inoculated with FAdV-8a strain T8-A had 30% IBH mortality by seven days of age.The hexon gene loop 1 sequence of the virus isolated from affected broiler progeny showed 100% identity to FAdV-8a strain T8-A.In order to demonstrate protection of broilers against IBH by vaccination of their parents, four groups of broiler breeders were vaccinated with either FAdV-8a strain T8-A (2x10 7 or 2x10 4 iii CCID 50 ) formulated with 20% oil-in-water emulsion, or FAdV x11a-like virus (2x10 7 or 2x10 4 CCID 50 ) formulated with 20% oil-in-water emulsion at the age of 12 and 15 weeks.The control group was inoculated with 20% oil-in-water emulsion.Broiler progeny were challenged with FAdV-8a strain T8-A to study the immunoprotective effect of the vaccine.Although, survival of broilers following FAdV-8a strain T8-A challenge was not significantly different among vaccinated and non-vaccinated groups (P>0.05),immunoprotective effect was enhanced by the increase dose of FAdV antigens (P>0.05).Further studies are necessary to improve the vaccine efficacy to protect broilers against IBH.In conclusion, the results of this study support the hypothesis that IBH in broilers in Canada is a vertically-transmitted primary disease with no known immunosuppressive involvement.The results also demonstrated that inactivated antigens of FAdV are able to partially protect broilers against IBH by vaccinating their parents.Further studies with different formulations, and priming the immune system of broiler breeders with live FAdV prior to vaccination with inactivated FAdV vaccines are necessary to improve the efficacy of inactivated IBH vaccine.and patience, and opportunity provided to me to pursue in graduate studies.I also bear gratitude to my graduate committee, Drs.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".