<i>Colletotrichum</i> <i>gloeosporioides</i> infection induces differential expression of glutathione S-transferase genes in <i>Malva</i> <i>pusilla</i>
Bibliographic record
Abstract
Among a collection of 840 expressed sequence tags of Malva pusilla leaves infected with Colletotrichum gloeosporioides f. sp. malvae (Cgm), a total of four different glutathione S-transferase (GST) (EC 2.5.1.18) genes were identified, each showing a different pattern of expression following infection. MpGSTU1 and MpGSTU2 were members of the class tau GSTs, MpGSTF1 was a member of the class phi GSTs, and MpGSTZ1 belonged to the class zeta GSTs. Infection by Cgm occurs by a hemibiotrophic process with an initial biotrophic phase preceding the necrotrophic phase and the appearance of symptoms. Expression of MpGSTZ1 progressively increased during infection, corresponding directly with the growth of the pathogen. Expression of MpGSTU2 was similar to that of MpGSTZ1, except for a greater increase during the late necrotrophic phase. MpGSTU1 expression remained relatively constant throughout the infection, whereas MpGSTF1 expression was induced primarily during the conversion from the biotrophic to necrotrophic phases of infection. Incubation of healthy mallow leaves in the dark resulted in decreased expression of MpGSTF1 and MpGSTU1, but not MpGSTZ1 and MpGSTU2. The differential expression patterns indicate that these mallow GST genes play a variety of roles in healthy and fungal-infected leaf tissue. The nucleotide sequences reported in this paper have been submitted to GenBank under the accession numbers AY206003, AY206001, AY206002, AY206000 and AY205999.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".