Blood‐brain barrier‐specific properties of a human adult brain endothelial cell line
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
Abstract
Establishment of a human model of the blood-brain barrier has proven to be a difficult goal. To accomplish this, normal human brain endothelial cells were transduced by lentiviral vectors incorporating human telomerase or SV40 T antigen. Among the many stable immortalized clones obtained by sequential limiting dilution cloning of the transduced cells, one was selected for expression of normal endothelial markers, including CD31, VE cadherin, and von Willebrand factor. This cell line, termed hCMEC/D3, showed a stable normal karyotype, maintained contact-inhibited monolayers in tissue culture, exhibited robust proliferation in response to endothelial growth factors, and formed capillary tubes in matrix but no colonies in soft agar. hCMEC/D3 cells expressed telomerase and grew indefinitely without phenotypic dedifferentiation. These cells expressed chemokine receptors, up-regulated adhesion molecules in response to inflammatory cytokines, and demonstrated blood-brain barrier characteristics, including tight junctional proteins and the capacity to actively exclude drugs. hCMEC/D3 are excellent candidates for studies of blood-brain barrier function, the responses of brain endothelium to inflammatory and infectious stimuli, and the interaction of brain endothelium with lymphocytes or tumor cells. Thus, hCMEC/D3 represents the first stable, fully characterized, well-differentiated human brain endothelial cell line and should serve as a widely usable research tool.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- The FASEB Journal
- Topic
- Barrier Structure and Function Studies
- Field
- Neuroscience
- Canadian institutions
- —
- Funders
- National Heart, Lung, and Blood InstituteOpen Society FoundationsUniversité Paris DescartesInstitut National de la Santé et de la Recherche MédicaleLeverhulme TrustMultiple Sclerosis Society of CanadaSidactionFondation pour l'Aide à la Recherche sur la Sclérose en PlaquesMultiple Sclerosis SocietyWellcome TrustCentre National de la Recherche ScientifiqueWellcome
- Keywords
- Blood–brain barrierEndotheliumCell biologyCD31Endothelial stem cellBiologyChemokineCell cultureImmunologyAngiogenesisCancer researchInflammationNeuroscienceCentral nervous systemIn vitroGenetics
- Has abstract in OpenAlex
- yes