Size-dependent PCR inhibitory effect induced by gold nanoparticles
Bibliographic record
Abstract
In this work, the effect of gold nanoparticles (AuNPs) with diameters of around 5, 10 and 20 nm on PCR efficiency is evaluated respectively using a real-time PCR machine. Gold nanoparticles show no obvious effect on PCR at low particle concentration. When the concentration is increased, PCR inhibition is observed. At the same particle concentration, gold nanoparticles of different sizes show different inhibitory effects on PCR. It is found that Taq polymerase can interact with AuNPs. The interaction is probably due to the binding of polymerase to AuNPs therefore lowering the concentration of free polymerase. It is also found that bovine serum albumin can interact with gold nanoparticles. It is believed that BSA blocks the surface of AuNPs from forming biding sites for polymerase. It can be used as an additive to reverse the inhibitory effect caused by gold nanoparticles.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".