Detection of the Aleutian mink disease virus DNA and antiviral antibodies in experimentally infected mink 10 days post-inoculation
Bibliographic record
Abstract
Black (n=44) and sapphire (n=12) mink were injected intraperitoneally with 10% spleen homogenates containing a local strain of the Aleutian mink disease virus (AMDV). Animals were euthanized 10 days post-inoculation (pi) and samples of blood and seven organs were collected. Viral DNA was extracted from blood plasma and cell-free organ homogenates (25% W/V) by magnetic beads and tested by PCR. Anti-viral antibodies in blood plasma were tested by counterimmunoelectrophoresis (CIEP). The virus was present in the spleen, lymph nodes, bone marrow and lung samples of all inoculated mink, but was not detected in some intestine (1.9%), kidney (10.7%) and liver (35.7%) samples. Viral DNA was detected in plasma of all inoculated mink by PCR, although two of the samples showed inconclusive test results. Antiviral antibodies were detected in plasma of three sapphire (25.0%) and 19 black (43.2%) mink. The relative specificity and sensitivity of the CIEP test on plasma samples were 100% and 39.3%, respectively, implying that low levels of antibodies during early stages of infection resulted in false negative CIEP tests. Histopathology revealed some lesions characteristics of AMDV infection in the liver and/or kidneys of three black and one sapphire mink, but heart and lung tissues had no detectible lesions. The results suggested that PCR was more reliable than CIEP for viral detection in the early period after infection, and that there were considerable differences among mink of each color type for production of detectable levels of anti-viral antibodies and development of disease symptoms. Samples of saliva, rectal swabs and feces collected on day 10 pi did not produce consistent and reliable results for viral detection by PCR.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".