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Record W2114564141 · doi:10.1002/ange.201308584

Umkodierung des genetischen Codes mit Selenocystein

2013· article· de· W2114564141 on OpenAlexaff
Markus J. Bröcker, Joanne M. L. Ho, George M. Church, Dieter Söll, Patrick O’Donoghue

Bibliographic record

VenueAngewandte Chemie · 2013
Typearticle
Languagede
FieldNursing
TopicSelenium in Biological Systems
Canadian institutionsWestern University
Fundersnot available
KeywordsMolecular biologyPhysicsBiologyPhilosophyChemistry

Abstract

fetched live from OpenAlex

Abstract Der Selenocystein(Sec)‐Einbau in Proteine erfolgt in der Natur durch die kotranslationale Umkodierung eines UGA‐Stopp‐Codons. Diese Studie zeigt nun, dass Sec nicht ausschließlich durch UGA, sondern vielmehr durch 58 aller 64 möglichen Codons kodiert werden kann. Hierbei erlauben 15 Codons nicht nur die vollständige Umkodierung von ihrer ursprünglichen Bedeutung als kanonische Aminosäure zu Selenocystein, sondern sie führen zu Proteinausbeuten, die um mehr als das Zehnfache gesteigert sind. Der hoch effiziente Mechanismus zur Selenocystein‐Rekodierung wird anhand zweier Reporterenzyme, der Escherichia‐coli‐ Formiatdehydrogenase und der humanen Thioredoxinreduktase, beschrieben. Da der Selenocysteineinbau an der Position eines UGA‐Stopp‐Codons zwangsläufig mit translationaler Termination konkurriert, war es umso erstaunlicher, dass die Selenocystein‐Einbaumaschinerie während der Umkodierung von Sense‐Codons erfolgreich mit den im Überfluss vorhandenen regulären Aminoacyl‐tRNAs konkurriert.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesInsufficient payload (model declined to judge)
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.113
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.009

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.030
GPT teacher head0.254
Teacher spread0.224 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; both teacher heads agree on what is shown here.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations9
Published2013
Admission routes1
Has abstractyes

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