Western blot: Technique, theory, and trouble shooting
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Machine scores (provisional)
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- Teacher spread
- 0.279 · how far apart the two teachers sit on this one work
- Validation status
score_only:v0-immature-baseline· verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it
Abstract
Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- North American Journal of Medical Sciences
- Topic
- Enzyme Structure and Function
- Field
- Materials Science
- Canadian institutions
- McMaster University
- Funders
- —
- Keywords
- Western blotBlotTroubleshootingTrouble shootingComputer scienceComputational biologyData scienceCell biologyBiologyBiochemistryEngineering
- Has abstract in OpenAlex
- yes