Transgenic <i>Brassica napus</i> plants overexpressing aluminium‐induced mitochondrial manganese superoxide dismutase cDNA are resistant to aluminium
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Full frame distilled prediction
Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
- Candidate categories
- Insufficient payload (model declined to judge)
- Consensus categories
- none
- Domain
- Candidate signal: noneConsensus signal: none
- Study design
- Candidate signal: Bench or experimentalConsensus signal: Bench or experimental
- Genre
- Candidate signal: EmpiricalConsensus signal: Empirical
- Teacher disagreement score
- 0.458
- Threshold uncertainty score
- 1.000
- Validation status
machine_predicted_unvalidated·codex-gemma-dda1882f352a
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
- Teacher spread
- 0.173 · how far apart the two teachers sit on this one work
- Validation status
score_only:v0-immature-baseline· verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it
Abstract
Abstract We investigated the role that manganese superoxide dismutase (MnSOD, EC 1·15·1·1), an important enzyme of the antioxidant pathway, may play in aluminium (Al) toxicity/resistance. A wheat ( Triticum aestivum ) cDNA ( WMnSOD ) was cloned and up‐regulation of the transcript was observed in root tips after 24 h exposure to 100 µ m Al. The WMnSOD1 under the control of the CaMV 35S promoter was expressed in canola ( Brassica napus ). Transgenic plants were phenotypically normal. Northern analyses showed enhanced levels of the WMnSOD1 and WMnSOD1‐GUS transcripts and total SOD activity was 1·5‐ to 2·5‐fold greater in transgenic plants than in wild type (WT) plants. Transgenic (T 1 ) leaf discs showed increased retention of chlorophyll and reduced electrolyte leakage when exposed to methyl viologen (MV) as compared with WT leaf discs, suggesting that transgenic plants were more resistant to oxidative stress. When WT canola plants were exposed to aluminium (0–200 µ m ), inhibition of root growth, higher SOD activity and increased levels of malondialdehyde (MDA, an indicator of lipid peroxidation) were observed in roots. Aluminium‐induced inhibition of root growth and accumulation of MDA was lower in homozygous transgenic plants (T 2 ) compared with WT plants. Transgenic lines also showed lower synthesis of 1,3‐ β ‐glucans (callose, a sensitive marker for Al injury) as compared with WT. These data suggest that resistance to Al toxicity can be improved by overexpressing WMnSOD1 .
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- Plant Cell & Environment
- Topic
- Aluminum toxicity and tolerance in plants and animals
- Field
- Agricultural and Biological Sciences
- Canadian institutions
- University of Alberta
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Keywords
- Superoxide dismutaseMalondialdehydeCanolaBrassicaCalloseTransgeneBiologyLipid peroxidationGenetically modified cropsReactive oxygen speciesAntioxidantHorticultureBiochemistryBotanyCell wallGene
- Has abstract in OpenAlex
- yes