Expression of porcine jejunal excitatory amino acid carrier 1 (EAAC1) and glutamate transporter associated protein 3‐18 (GTRAP3‐18) during postnatal development
Bibliographic record
Abstract
The excitatory amino acid carrier 1 (EAAC1) is expressed in both the small bowel and the central nervous system and is responsible for the homeostasis of the neurotransmitter L‐glutamate. Glutamate transporter associated protein 3‐18 (GTRAP3‐18) is an inhibitory regulator of EAAC1 by affecting its trafficking and through protein‐protein interactions. We examined jejunal EAAC1 protein and mRNA abundances and GTRAP3‐18 protein abundance during the postnatal development in pigs at the ages of d 1, 4, 6, 12, 20, 28 (1‐wk post‐weaning), and 70 (mature gut), respectively. There were linear decreases ( P <0.05) in the jejunal EAAC1 and GTRAP3‐18 protein abundances in homogenate, cytosol and the apical membrane, whereas a cubic pattern was observed ( P <0.05) in the jejunal EAAC1 mRNA abundance during postnatal growth. Furthermore, EAAC1 protein abundances were correlated with GTRAP 3‐18 protein abundances in homogenate (r=0.92, P <0.01), cytosol (r=0.91, P <0.01) and the apical membrane (r=0.81, P <0.01), respectively. However, the EAAC1 protein abundances in jejunal homogenate, cytosol and the apical membrane were poorly correlated ( P >0.05) with jejunal EAAC1 mRNA abundance. These results suggest that there is a postnatal decrease in the jejunal EAAC1 expression likely regulated at the post‐transcriptional level. A parallel decline in GTRAP3‐18 protein abundance may further reduce EAAC1 function in the jejunum during postnatal growth. Supported by NSERC Discovery Program of Canada.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".