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Record W2117278538 · doi:10.1095/biolreprod.110.088864

Techniques for Culturing Spermatogonial Stem Cells Continue to Improve

2010· review· en· W2117278538 on OpenAlexafffundabout
Makoto Nagano

Bibliographic record

VenueBiology of Reproduction · 2010
Typereview
Languageen
FieldMedicine
TopicSperm and Testicular Function
Canadian institutionsMcGill UniversityRoyal Victoria Hospital
FundersMcGill University
KeywordsBiologyStem cellSpermatogenesisTransplantationEmbryonic stem cellCell biologyAndrologyRegeneration (biology)Fetal bovine serumFetusIn vitroImmunologyInternal medicineGeneticsEndocrinologyGenePregnancy

Abstract

fetched live from OpenAlex

In this issue of Biology of Reproduction, Kanatsu-Shinohara et al. [1] report a novel technique for culturing spermatogonial stem cells (SSCs) in the absence of both serum and feeder cells. This achievement has significant implications in SSC research and applications. To appreciate this study, it is useful to briefly review the past progress in the development of SSC culture systems. The study of SSCs dates back to the 1950s [2]; however, functional analyses of SSCs were limited until 1994, when the group led by Ralph Brinster demonstrated the regeneration of complete spermatogenesis following transplantation of donor mouse testis cells into recipient testes [3, 4]. Because stem cells are defined by their ability to reconstitute an adult tissue [5], this transplantation technique provided an unequivocal detection method of SSC potential. Nonetheless, the technique alone did not allow for the experimental manipulation of SSCs or for the dissection of their cellular biology. The SSC culture was therefore expected to become an important tool in understanding the mechanisms that control SSC survival, self-renewal, proliferation, and differentiation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.006
metaresearch head score (Gemma)0.005
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.007
Threshold uncertainty score0.029

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0060.005
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0030.002
Science and technology studies0.0010.002
Scholarly communication0.0040.004
Open science0.0020.002
Research integrity0.0020.006
Insufficient payload (model declined to judge)0.0070.010

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.330
Teacher spread0.295 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations13
Published2010
Admission routes3
Has abstractyes

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