Development and characterization of polymorphic microsatellite markers for <i>Conopholis americana</i> (Orobanchaceae)
Bibliographic record
Abstract
PREMISE OF THE STUDY: Conopholis americana is an obligate root parasite with highly reduced morphology. To investigate the population structure, genetic diversity, and mating system of this predominantly eastern North American species, we developed polymorphic microsatellite markers for C. americana. METHODS AND RESULTS: Using an enrichment cloning protocol, we report the isolation and characterization of 11 microsatellite markers. Product size varied from 198-370 bp. These loci show moderate levels of allelic variation (averaging 4.182 alleles per locus) and very low levels of heterozygosity (average observed heterozygosity = 0.054). CONCLUSIONS: These microsatellite markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. americana.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".