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Record W2118295931 · doi:10.1080/10495390009525947

Expression of x inactive specific transcript<i>(XZST)</i>and testicular morphogenesis in bovine fetuses

2000· article· en· W2118295931 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueAnimal Biotechnology · 2000
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsXISTBiologyX-inactivationDosage compensationFetusGene expressionX chromosomeAndrologyGeneProphaseMolecular biologyGeneticsCell biologyMeiosisPregnancy

Abstract

fetched live from OpenAlex

Inactivation of one of the 2 X chromosomes in the somatic cells of female mammals is the process by which their X-linked gene products are equalized to those of their male counterparts. In male mammals, however, a sex vesicle representing the condensed and transcriptionally silenced sex chromosomes is detected during early meiotic prophase. Since the exact stage of development at which X inactivation is initiated in the bovine testis is not established as yet, we undertook to study fetuses ranging in age from 30 to 180 days of gestation, to determine the transcriptional status of the Xist gene currently thought to be the prerequisite component of X inactivation. Our studies using reverse transcription polymerase chain reaction (RT-PCR) approach with primers designed to amplify a 463 bp product from a conserved region of the first exon of bovine Xist gene, proved that Xist expression is evident in bovine fetal testes as early as 50 days of gestation and that it continues at least to the end of the second trimester (180 days) of gestation. Morphological studies on fetal testes during gestational stage spanning the period of Xist expression revealed the presence of large intra-tubular cells overtly resembling the prespermatogonia of postnatal bovine testes, at 50 days and preleptotene like cells as early as 90 days of gestation. We hypothesize that the expression of the Xist gene, or the recently discovered Tsix gene antisense to Xist in orientation, may be related to the presence of these cells which participate in the morphogenesis of the fetal bovine testis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.019
Threshold uncertainty score0.508

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.228
Teacher spread0.219 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it