Cloning and Characterization of a Plasma Membrane H<sup>+</sup>-ATPase (PMA) Gene from a Salt-tolerant Plant <i>Chloris virgata</i>
Bibliographic record
Abstract
A plasma membrane H + -ATPase (PMA) gene ( ChvPMA ) was isolated from a wild salt-tolerant plant Chloris virgata . The expression of ChvPMA gene in leaves and roots of Chloris virgata seedlings under salt stress (NaCl, NaHCO 3 ) was examined. The results showed the ChvPMA gene expression was induced by salt stress. The ChvPMA gene was fused to the N-terminus of green fluorescence protein (GFP) gene, and transferred into onion epidermal cells for analyses of intracellular localization. The result showed that the ChvPMA protein was found to be in the plasma membrane of ion epiderm. Because the H + -ATPase activity is regulated by a C-terminal auto-inhibitory domain that can be displaced by phosphorylation, we analyzed transgenic yeast expressing either wild-type PMA ( ChvPMA ) or truncated ChvPMA lacking the C-terminal auto-inhibitory domain ( ChvPMAΔC ) under high salt and pH conditions. The results showed that over-expression of ChvPMA and ChvPMAΔC in transgenic yeasts increased the resistance to salt and lower pH conditions, especially, the yeast over-expressing ChvPMAΔC showed better growth than ChvPMA at an external pH 4.0 in the presence NaCl. Transgenic Arabidopsis over-expressing ChvPMAΔC also showed the better root growth than that of ChvPMA at an external pH 4.0 in the presence NaCl.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".