Plant pyruvate-dependent gamma-aminobutyrate transaminase: identification of an<i>Arabidopsis</i>cDNA and its expression in<i>Escherichia coli</i>
Why this work is in the frame
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Bibliographic record
Abstract
Both pyruvate- and 2-oxoglutarate-dependent gamma-aminobutyrate transaminase (GABA-T) activities are present in crude tobacco (Nicotiana tabacum L.) leaf extracts. In this study, GABA:pyruvate-T activity was partially purified using mitochondrial isolation and protein solubilization in 3-[3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, and a combination of chromatographic and electrophoretic procedures. A partial amino acid sequence of the putative 55-kDa GABA-T subunit enabled identification of a predicted Arabidopsis thaliana (L.) Heynh. GABA:pyruvate-T expressed sequence tag and subsequent amplification of a 1515 bp open reading frame encoding a 504-amino acid polypeptide. Computer analysis using web-based tools revealed the presence of a putative mitochondrial signal sequence and a pyridoxal-5-phosphate binding domain in the polypeptide. Functional expression of the GABA-T cDNA in Escherichia coli revealed that the recombinant protein uses pyruvate but not 2-oxoglutarate. The Arabidopsis GABA:pyruvate-T cDNA could form the basis for identification of multiple GABA-T isoforms and generation of GABA-T mutants for determining the fate of GABA nitrogen and elucidating the physiological function of GABA in plants.Key words: amino acceptor, gamma-aminobutyrate, gamma-aminobutyrate transaminase, protein purification, heterologous expression, recombinant protein.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it