Effect of embryo orientation on the developmental sequence of adventitious organogenesis in jack pine (<i>Pinus banksiana</i> Lamb.)
Bibliographic record
Abstract
The developmental sequence of adventitious organogenesis in jack pine (Pinus banksiana Lamb.) embryos was analysed depending on two explant orientations. Embryos were placed vertically (cotyledons downward) or horizontally on half-strength Schenk and Hildebrandt medium containing 10 µM 6-benzylaminopurine. Samples were harvested at 2- to 3-day intervals for a 21-day period and observed using light and electron microscopy. In vertically placed embryos, cotyledons were inserted into the medium and became necrotic, while buds and phylloids developed on the upper part of the hypocotyl and in the apical area, 14 and 9 days after culture initiation respectively. In horizontally cultured embryos, organogenesis started in the intercotyledonary areas (day 11) and spread to the abaxial surface of cotyledonary margins outside the medium (day 14). A few buds also developed on the adaxial surface of cotyledons, starting on day 16. In the apical area, phylloid formation started on day 18. Nodules were induced in tissues in contact with the medium for both orientations, but in distinct sites. Embryo orientation influenced the localization of induction sites and the time sequence of organogenesis. The pattern for the mobilization of proteins, lipids, and polysaccharides prior to organogenesis was similar for both orientations. A depletion in proteins and polysaccharides did not occur in tissues in contact with the culture medium.Key words: adventitious organogenesis, embryo culture, explant orientation, jack pine, microscopy, Pinus banksiana.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".