Identification of esterase in<i>Aspergillus flavus</i>during degradation of polyester polyurethane<sup>1</sup>
Why this work is in the frame
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Bibliographic record
Abstract
Polyurethane not only has applications including tires and insulation, but also is an essential element of our lives. Based on previous reports, some strains of fungus including Pestalotiopsis microspora have been reported to degrade this plastic. The ultimate objective is to create a genetically engineered variant of Pichia pastoris which will digest polyester polyurethane (PUR) using the enzyme responsible for PUR degradation from Aspergillus flavus ; this year we aim to identify the esterase, believed to be responsible for the biodegradation. The degradation of TPU (a granular form of thermoplastic PUR) and water-based PUR by A. flavus , was tested using incubation with shaking for one month. Although no significant weight loss was observed, the fungus was capable of growing on the water-based PUR as a food source. Esterase, the enzyme responsible for the degradation of the water-based PUR, was observed in the water-based PUR lane of the Native PAGE gel and has a molecular weight of approximately 20 kDa. Since the only difference between the experimental water-based lane and experimental TPU lane, was the esterase band, it can be confirmed that the degradation of the plastic and growth of fungus can be attributed to the esterase. Le polyuréthane a non seulement des applications dans la fabrication des pneus et pour l’isolation, mais est aussi un élément essentiel à notre vie. Conformément à des rapports antécédents, certaines souches de mycète, dont Pestalotiopsis microspora , ont su démontrer la dégradation du plastique. L’objectif ultime est la création d’un mutant du Pichia pastoris par génie génétique qui pourra digérer le polyester polyéthane (PUR) en utilisant l’enzyme responsable de la dégradation du PUR de l’Apergillus flavus ; cette année, nous visons à identifier l’estérase, soupçonnée d’être responsable de la biodégradation. La dégradation du TPU (une forme granulaire du PUR thermoplastique) et du PUR à base d’eau par A. flavus furent testées par incubation avec agitation pendant une durée d’un mois. Malgré aucune perte de poids significative observée, le mycète fut capable de croitre sur les PUR à base d’eau comme source alimentaire. L’estérase, l’enzyme responsible de la dégradation des PUR à base d’eau, fut observé dans le puits avec du PUR à base d’eau du gel PAGE (électrophorèse sur gel polyacrylamide) Native et a une masse moléculaire d’environ 20kDa. Étant donné que la seule différence entre le puits expérimental à base d’eau et le puits expérimental TPU était la bande d’estérase, on peut confirmer que la dégradation du plastique et la croissance du mycète peuvent être attribuées à l’estérase.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it