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Arabidopsis Seed Coat Mucilage is a Specialized Cell Wall that Can be Used as a Model for Genetic Analysis of Plant Cell Wall Structure and Function

2012· article· en· 139 citations· W2124515732 on OpenAlex· 10.3389/fpls.2012.00064

Why is this work in the frame?

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

Canadian affiliationAn author listed a Canadian institution. This is the only route the usual frame has.

Full frame distilled prediction

Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

Candidate categories
none
Consensus categories
none
Domain
Candidate signal: noneConsensus signal: none
Study design
Candidate signal: Bench or experimentalConsensus signal: none
Genre
Candidate signal: EmpiricalConsensus signal: Empirical
Teacher disagreement score
0.558
Threshold uncertainty score
0.434
Validation status
machine_predicted_unvalidated · codex-gemma-dda1882f352a

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Opus teacher head0.021
GPT teacher head0.203
Teacher spread
0.182 · how far apart the two teachers sit on this one work
Validation status
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Abstract

Arabidopsis seed coat epidermal cells produce a large quantity of mucilage that is extruded upon exposure to water. Chemical analyses and cell biological techniques suggest that this mucilage represents a specialized type of secondary cell wall composed primarily of pectin with lesser amounts of cellulose and xyloglucan. Once extruded, the mucilage capsule has a distinctive structure with an outer non-adherent layer that is easily removed by shaking in water, and an inner adherent layer that can only be removed with strong acid or base. Most of the cellulose in the mucilage is present in the inner layer and is responsible at least in part for its adherence to the seed. There are also differences in the pectin composition between the two layers that could contribute to the difference in adherence. The Arabidopsis seed coat epidermis and its mucilage are not essential for seed viability or germination. This dispensability, combined with the fact that the epidermal cells synthesize an accessible pectin-rich cell wall at a specific time in development, makes them well suited as a genetic model for studying cell wall biogenesis, function, and regulation. Mutants defective in seed mucilage identified by both forward and reverse genetic analyses are proving useful in establishing connections between carbohydrate structure and cell wall properties in vivo. In the future, genetic engineering of seed coat mucilage carbohydrates should prove useful for testing hypotheses concerning cell wall structure and function.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

The record

Venue
Frontiers in Plant Science
Topic
Polysaccharides and Plant Cell Walls
Field
Agricultural and Biological Sciences
Canadian institutions
McGill UniversityUniversity of British Columbia
Funders
not available
Keywords
MucilageCell wallXyloglucanArabidopsisSecondary cell wallPectinArabidopsis thalianaBiologyPolysaccharideEpidermis (zoology)CoatCelluloseBotanyGerminationPlant cellMutantBiophysicsCell biologyBiochemistryGeneAnatomy
Has abstract in OpenAlex
yes