Application of Phase Images for Estimation of Peritoneal Macrophages State
Why this work is in the frame
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Bibliographic record
Abstract
Procedure based on phase images obtained by the method of Laser Interference Microscopy (LIM) for detection of number non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro was provided. The phase images provide quantitative estimates of the optical path difference (OPD) in each point of object. The OPD varies with cell thickness and concentration of cellular substances. Therefore, it provides useful information on cell structure, localization of subcellular structures, local concentrations of substances and dry mass of cell. In these work we propose a new assay for estimation a number of non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro based on cell phase images. The results were compared with phagocytic index, which was evaluated using latex beads, and the percentage of cells with damaged membranes (dead cells). It was shown that with increasing time after the isolation, the percentage of dead macrophages estimated by LIM was similar to that of the cells with damaged membranes. However, the percentage of activated cells calculated using latex beads exceeded the percentage calculated using the phase images significantly.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it