Isolation of Arachis hypogaea Na+/H+ antiporter and its expression analysis under salt stress
Why this work is in the frame
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Bibliographic record
Abstract
The plant Na+/H+ antiporter gene plays a major role in salt tolerance. One Na+/H+antiporter (AhNHX1) gene was isolated from peanut (Arachis hypogaea) in the present work. The full-length cDNA of AhNHX1 was 2,331 bp, which contains a complete ORF of 1,620 bp. The deduced protein sequence contains 546 amino acids with a molecular mass of 58.8 KDa and a pI of 7.23. The amino acid sequence of the AhNHX1 shares high similarity (shows more than 81.89% identity) with those of the previously isolated NHX1 from other plants. Meanwhile, theAhNHX1 protein has twelve putative transmembrane domains, and the conserved amiloride-binding sites (84LFFIYLLPPI93) were found in its N-terminal. Also, the expression of AhNHX1 was tissue-specific under different level NaCl treatments. Under 50 and 100 mM NaCl treatments, its expression showed higher in roots and lower in stems and leaves relative to control plants, respectively. In addition, its expression was time-specific, such that under 150 mM NaCl treatment, its expression usually increased and reached to a stable level in roots and leaves after 24 h but in stems after 48 h, respectively. These results implied that the AhNHX1plays an important role under salt stress in peanut. Key words: Arachis hypogaea, AhNHX1, salt tolerance, gene expression patterns.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it