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<i>Apis mellifera</i> has two isoforms of cytoplasmic HSP90

2010· article· en· W2137363659 on OpenAlex
Pengjun Xu, Jin Hua Xiao, Qun Xia, Bruce D. Murphy, Dahui Huang

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueInsect Molecular Biology · 2010
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicInsect and Pesticide Research
Canadian institutionsRoyal Ontario Museum
FundersNational Natural Science Foundation of China
KeywordsBiologyGene isoformCytoplasmHsp90Cell biologyComputational biologyHeat shock proteinGeneticsGene

Abstract

fetched live from OpenAlex

Unlike most other insects, annotated genomic data suggest that Apis mellifera has two homologous copies of the cytoplasmic gene HSP90. In this study, we did a phylogenetic analysis on these two copies with some other insects HSP90 genes, and we also manipulated a reverse transcript (RT)-PCR to find all the putative transcripts for both copies. Phylogenetic analysis indicates that A. mellifera possesses two isoforms of cytoplasmic HSP90: The 'traditional' isoform clusters with cytoplasmic HSP90 of other insects. The other isoform, which occurs phylogenetically as the sister group of all insects, may be a new gene and specific to A. mellifera. The results of RT-PCR indicate that this new isoform contains at least eight transcripts derived from the same genomic locus by complicated alternative splicing (GenBank accession numbers: FJ713701, FJ713702, FJ713703, FJ713704, FJ713705, FJ713706, FJ713707 and FJ713708, respectively). The existence of this specific HSP90 might be related to the caste differentiation of bees.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.014
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.026
GPT teacher head0.278
Teacher spread0.252 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it