Certificación de la cadena de custodia en el sector de la madera: La experiencia de MARIANO HERVÁS S.A
Bibliographic record
Abstract
AdeFGH is a multidrug resistance efflux pump of Acinetobacter baumannii belonging to the resistance-nodulation-cell division (RND) family. Analysis of expression of this pump established it as the most prevalent RND pump in clinical isolates of A. baumannii from Canadian hospitals. In this study, the activity of the efflux pump inhibitor (EPI) phenylalanine-arginine β-naphthylamide (PAβN) against the RND efflux pump AdeFGH of A. baumannii was analysed. The adeFGH operon was cloned under the control of the tac promoter and was introduced in single copy into a surrogate Pseudomonas aeruginosa strain that is devoid of its six native efflux pumps. Isopropyl-β-D-thiogalactopyranoside (IPTG) was used to induce expression of the pump and the sensitivity of strains expressing the adeFGH operon to PAβN was analysed in the surrogate strain and in clinical isolates of A. baumannii by measuring the minimum inhibitory concentrations of trimethoprim, chloramphenicol and clindamycin (substrates of AdeFGH) in the presence and absence of PAβN. PAβN was found to inhibit the ability of the AdeFGH pump to efflux all three antibiotics tested in the surrogate P. aeruginosa strain as well as in the A. baumannii strains. However, in A. baumannii strains the inhibition was more profound for clindamycin than for trimethoprim and chloramphenicol. In addition, these data also support the importance of a strain devoid of efflux pumps for screening of EPIs, since the inhibition of AdeFGH by PAβN was found to be more effective in the surrogate P. aeruginosa strain than in the clinical isolates of A. baumannii.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.006 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".