Immersion in Neutral Red Solution as a Mass-Marking Technique to Study the Movement of the Amphipod Corophium Volutator
Why this work is in the frame
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Bibliographic record
Abstract
We evaluated the usefulness of immersion in neutral red solution as a marking technique for studies of movement of the amphipod Corophium volutator. Amphipods were immersed for 20 minutes in solutions of neutral red at different concentrations (25, 50 and |$100\, {\text{mg L}}^{- 1}$|). Amphipods incorporated dye at all concentrations, and the hepatopancreas remained distinctly stained red for at least 7 days. No increase in mortality was detected at any concentration when compared to a control treatment |$(0\, {\text{mg L}}^{- 1})$|. In a separate field experiment, staining did not significantly affect tendency of C. volutator to move. Overall, immersion in neutral red seems to be an appropriate technique to rapidly mark large numbers of C. volutator and is suitable for short-term mark-recapture experiments. This technique may also apply to other small-sized crustacean species with relatively transparent exoskeletons.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it