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Record W2142777242 · doi:10.1093/jxb/erp203

Germination of Arabidopsis thaliana seeds is not completed as a result of elongation of the radicle but of the adjacent transition zone and lower hypocotyl

2009· article· en· W2142777242 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueJournal of Experimental Botany · 2009
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicSeed Germination and Physiology
Canadian institutionsUniversity of Guelph
FundersNatural Sciences and Engineering Research Council of CanadaOregon State University
KeywordsRadicleHypocotylGerminationElongationArabidopsis thalianaEndospermBiologyBotanyColeoptileArabidopsisCell biologyBiochemistryGene

Abstract

fetched live from OpenAlex

The completion of germination of seeds of Arabidopsis thaliana is marked by the appearance of the radicle through the surrounding endosperm and testa. Using confocal microscopy and green fluorescent protein (GFP)-transformed embryos to highlight the epidermal cell walls it has been possible to conduct time-lapse photography of individual embryos during their germination. This reveals that the elongation of embryo cells to effect completion of germination does not occur within the radicle itself, but rather within a discrete region that is immediately proximal to the radicle. This region, identifiable as the lower hypocotyl and hypocotyl-radicle transition zone, is also definable by accumulation of carbohydrate-containing bodies during germination, and distinct GFP expression of GAL4-GFP in enhancer trap lines. Flow cytometric studies show that there is an increase in the proportion of 4C nuclei in the axis which coincides with a considerable increase in length of the hypocotyl, and the occurrence of endopolyploid (8C and 16C) nuclei accompanies the 2-fold increase in mean cell size in the region of elongation, the lower hypocotyl, and hypocotyl-radicle transition zone. Thus the observed cell elongation during germination is accompanied by an increase in nuclear DNA content, and the resultant elongation of the axis to effect radicle emergence is due to cell expansion, not to cell division. When studying the molecular events involved in the completion of germination, therefore, it may be prudent to focus on this region of elongation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.257
Threshold uncertainty score0.106

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.245
Teacher spread0.228 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it