Permeabilization of<i>Beauveria bassiana</i>blastospores for in situ enzymatic assays
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Vigorous agitation of an aqueous suspension of blastospores (BS) of Beauveria bassiana mixed with nine volumes of a 1:4 (v/v) mixture of toluene:ethanol (95%) for 10 min permits blastospore permeabilization. Agitation results in greater membrane permeabilization than heating blastospores in the presence of toluene:ethanol or the detergents Triton X-100, sodium dodecyl sulfate, hexadecyltrimethylammonium bromide and Brij-35. The β-galactosidase activity in permeabilized blastospores was determined with these methods. The effectiveness of permeabilization in detecting enzyme activity was assessed by comparison to whole BS lysates prepared by mechanical disruption and pressurized disruption of BS biomass. The toluene:ethanol method was applied to study the incorporation of (3)H-thymidine triphosphate into blastospore DNA. Whole BS permeabilization allows the examination of enzyme activity and DNA synthesis at a cellular level in this important mycoinsecticide.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it