Bacterial cultures capable of facultative growth on methane under thermophilic or thermotolerant conditions
Bibliographic record
Abstract
Bacteria cultured from thermal features (cultures OS and 44) and from sedimentary rock of the continental margin (SA) are capable of growth on methane as a sole carbon source under thermotolerant (OS and 44) to thermophilic (SA) conditions. All three grew better on enriched medium, suggesting methane is not a preferred substrate. Sequencing (16S rDNA) suggests OS and 44 are primarily Brevibacillus and SA is primarily Geobacillus , not members of described methanotrophic genera. These cultures appear pure based on cell morphology, however they may be consortia of tightly syntrophic bacteria. Growth on 13C-methane demonstrated that these bacteria incorporate carbon from methane. Labeling (δδ13C) was greatest when cultures were grown on methane only. When medium was supplemented with yeast extract (0.05%) labeling was less intense, however total methane incorporation was greater due to increased bacterial biomass. These bacterial cultures could have application for methane removal from waste streams at high temperatures.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".