Cysteinyl leukotrienes dependent [Ca2+]i responses to Angiotensin II in rat cardiomyocytes and aortic smooth muscle cells
Bibliographic record
Abstract
Angiotensin II (Ang II) plays a very important role in regulating cardiac and vascular contraction and proliferation/hypertrophy via stimulation of AT 1 receptors.A few studies have demonstrated that 5-lipoxygenase (5-LO) derived cysteinyl leukotrienes (CysLT) contribute to Ang II evoked tension responses in rat aortic rings.Whether CysLT would contribute to Ang II evoked Ca 2+ mobilization in neonatal rat cardiomyocytes (NRC) and rat aortic smooth muscle cells (ASMC) has not been investigated.In the present study, using primary cultures of NRC and minimally passaged cultures of rat ASMC, an effort was made to address this key issue.The agonists evoked increase in cytosolic free calcium ([Ca 2+ ] i ) level was determined by fura-2 fluorescence measurement in NRC and ASMC.Total CysLT levels in the culture medium were determined using an ELISA kit.CysLT 1 /CysLT 2 receptor mRNA levels of NRC and ASMC were quantified by Northern blot analysis.In NRC, the AT 1 but not the AT 2 selective antagonist, attenuated the elevations in [Ca 2+ ] i and CysLT levels evoked by Ang II.Vasopressin (AVP) and endothelin-1 (ET-1) increased [Ca 2+ ] i but not CysLT levels.The 5-LO inhibitor, AA861, and the CysLT 1 selective antagonist, MK-571, reduced the maximal [Ca 2+ ] i responses (E max ) to Ang II but not to AVP and ET-1.While CysLT 1 antagonist reduced the E max to leukotriene D 4 , (LTD 4 ), the dual CysLT 1 /CysLT 2 antagonist, BAY u9773, completely blocked the [Ca 2+ ] i elevation to both LTD 4 and leukotriene C 4 (LTC 4 ).Both CysLT 1 and CysLT 2 mRNA were detected in NRC.The inositol 1,4,5 triphosphate sincere thanks to
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.002 |
| Open science | 0.001 | 0.002 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".