Self-renewal and Proliferation of Murine Embryonic Stem Cells: A Study of Glycosaminoglycans Effect on Feeder-Free Cultures
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
The self-renewal and proliferation of murine embryonic stem (ES) cells can be preserved indefinitely in the presence of the mouse embryonic fibroblast (MEF) feeder layer. Since the feeder layer has several drawbacks, including viral contamination and large-scale production, gelatin solutions are the most prominent alternative to replacing it. In this investigation, self-renewal and proliferation of ES cells was carried out by supplementing the gelatin solution with glycosaminoglycan components, such as chondroitin sulfate and heparin. The methylcellulose-based embryoid body (EB) assay was used to evaluate the self-renewal ability of the ES cells. Chondroitin sulfate and heparin were mixed with 0.1% gelatin solution in 0:100, 50:50 and 100:0 (heparin/chondroitin sulfate) ratios and compared with 0.1% gelatin (positive control) and MEF (negative control) in three independent parallel experiments. Chondroitin sulfate addition to 0.1% gelatin enhanced the cell population and the number of EBs by 57 and 32%, respectively.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it