Pyruvate Kinase, Phosphoenolpyruvate Carboxykinase, Cytochrome C Oxidase and Catalase Activities in Cadmium Exposed<i>Perna viridis</i>Subjected to Anoxic and Aerobic Conditions
Bibliographic record
Abstract
This work concerns the effects of cadmium exposure on the anaerobic and oxidative metabolism in tissues of the hypoxia-tolerant green mussel Perna viridis from Venezuelan coastal waters. The mussels were exposed to different Cd concentrations (0, 20, 40 and 60 µg/L Cd) for 7 days, and were subsequently exposed to anoxia. Prior to anoxia exposure, Cd concentration was determined in the digestive gland and adductor muscle. After 24 h of anoxia, the activities of pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), and cytochrome c oxidase (CCO), and catalase (CAT) were measured in the digestive gland and adductor muscle from Cd-treated mussels. Also, effects of the Cd treatments on the median survival time (LT50) during anoxia were determined. The short-term Cd-exposed mussels augmented the metal uptake in the tested tissues in a dose-dependent manner, associated with a concomitant reduction in the ability to withstand anoxia. During normoxic and anoxic conditions, the Cd-cxposed individuals displayed decreased levels of PK, PEPCK, and CCO in comparison with controls, with the change being most evident during anoxia. Conversely, an increase in CAT was observed in Cd-treated organisms. This effect was further potentiated during anoxia. The toxic action of Cd uptake on PK and PEPCK could reduce the tolerance of anoxia, because these are regulatory glycolytic enzymes in mussels. Moreover, the decreased CCO activity could limit the capacity to recuperate from anoxia. The enhanced CAT activity in Cd-exposed organisms may have been in response to H2O2 produced during oxidative stress, and was likely accentuated during anoxia.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.002 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".