Viability of <i>Lactobacillus Rhamnosus</i> R0011 in an Apple‐Based Fruit Juice under Simulated Storage Conditions at the Consumer Level
Bibliographic record
Abstract
Lactobacillus rhamnosus R0011 was inoculated in an apple-pear-raspberry juice blend at 4.5 x 10(9) CFU/250 mL portion, and viability was followed during storage in conditions simulating consumer use. Study parameters were age of the juice (2 or 4 wk), sampling size (one to three 250-mL portions), incubation temperature (between 2 and 7 degrees C), and atmosphere (aerobic compared with anaerobic). Dependent variables were pH, dissolved oxygen (DO) level, and viable count. The DO level in the unopened PETE commercial bottles decreased from 10% to 3% over a 5-wk storage period at 7 degrees C. In shaken and opened bottles, DO increased to 15% during the 1st week of storage, irrespective of sampling size. However, agitation of bottles having more that 250 mL of headspace initially increased DO readings to 30% of maximum, but the DO level subsequently dropped to 15% during the 1-wk storage period between samplings. The presence of L. rhamnosus R0011 did not affect the DO data. The pH varied between 3.6 and 3.9, and was not influenced by L. rhamnosus R0011. However, the pH increased on average by 0.1 unit following multiple agitations and samplings. When the juices remained unopened in the PETE bottles, the viable population gradually dropped by 75% over 5 wk of storage at 7 degrees C, while in opened bottles reduction were only between 20% and 40%. Viability losses under anaerobic conditions were twice as high at 7 degrees C than at 4 degrees C. Data show that consumers can expect good viability of L. rhamnosus R0011 over a few weeks of storage in a refrigerator, even if the bottles have been opened and cells are exposed to oxygen.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".