Dietary ascorbic acid requirement of juvenile ayu (Plecoglossus altivelis)
Bibliographic record
Abstract
To investigate dietary ascorbic acid (AA) requirement of juvenile ayu (Plecoglossus altivelis) weighing 1.27 ± 0.02 g, eight diets were formulated with graded levels (0, 20, 40, 80, 160, 320, 640 and 1280 mg AA kg−1) of AA supplied as ascorbyl polyphosphate. Each experimental diet was fed to four-replicate groups to apparent satiation three times a day for 8 weeks. At the end of the feeding trial, fish fed AA-deficient diet showed visible AA deficiency signs and low survival. Based on the four-parameter saturation kinetics model, the calculated AA requirement levels for each dose-dependent response [weight gain, hepatic AA concentration, hydroxyproline (HyPro) concentration in skin and HyPro concentration in backbone] were 116, 226, 47 and 35 mg kg−1, respectively. Based on the maximal growth performance, a level of 116 mg AA kg−1 was recommended for commercial diet of juvenile ayu. To maintain tissue HyPro saturation and avoid AA deficiency symptoms, the minimum required dietary AA level was 47 mg kg−1. Hepatic AA saturation was considered as the most stringent criterion for determination of AA requirement.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".