Differentially Expressed Proteins of Soybean (Glycine max) Pulvinus in Light and Dark Conditions
Bibliographic record
Abstract
Some plant species both track and avoid the sun through turgor changes of the pulvinus tissue at the base of their leaves, maximizing light reception in dim conditions and minimizing cellular damage due to excessive light. Pulvinar response is known to be affected by both diurnally varying environmental factors and circadian patterns. Differential expression of the proteins between light and darkness are not well-known. In this study we used two-dimensional gel electrophoresis and mass spectrometry to separate and identify proteins in the soybean leaf pulvinus that were differentially expressed in the light compared to a dark control. Out of 165 protein spots previously identified (data not shown) 11 were found to have decreased expression in the light and 7 had increased light expression. The proteins that were more highly expressed in the light were mostly stress response proteins, while the under-expressed proteins were categorized as energy proteins. While the higher levels of expression of stress response proteins in the light align with other studies, the under-expressed light proteins require further examination to rule out artefactual results. These findings can provide a better understanding of the circadian pattern of protein expression in the legume pulvinus proteome.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".