<i>Escherichia coli</i> heat-stable enterotoxin b (STb) in vivo internalization within rat intestinal epithelial cells
Bibliographic record
Abstract
Heat-stable enterotoxin b (STb) is a low molecular weight toxin known to bind sulfatide, its receptor.The fate of STb bound to rat intestinal epithelium cells was followed using an anti-toxin gold labeled assay and transmission electron microscopy.The data suggest that STb toxin and the fusion protein maltose binding protein (MBP)-STb were internalized whereas its mutant I41E-M42R with reduced hydrophobicity did not show internalization.There was a significant difference in the mean of gold particles per field between rat intestine incubated with STb or the fusion protein MBP-STb and the negative control consisting of intestine incubated with PBS alone.No subcellular compartment seems to be particularly aimed by the toxin as gold particles were randomly distributed within the cell. Escherichia coli / STb enterotoxin / internalizationRésumé -Internalisation in vivo de l'entérotoxine b (STb) d'Escherichia coli dans les cellules épithéliales d'intestin de rat.L'entérotoxine b thermostable (STb) est une toxine de faible masse moléculaire qui se lie au sulfatide, son récepteur.À l'aide d'un essai avec un anticorps anti-STb marqué à l'or suivi d'une visualisation en microscopie électronique, nous avons observé les évènements suivant l'attachement de STb aux cellules épithéliales d'intestin de rat.La toxine STb et la protéine de fusion Maltose Binding Protein-STb (MBP-STb) ont été internalisées tandis que le mutant I41E-M42R avec une hydrophobicité réduite ne présentait pas d'internalisation.Nous avons observé une différence significative de la moyenne de particules d'or par champ entre l'intestin de rat incubé avec STb ou la protéine de fusion MBP-STb et le contrôle négatif, soit le tampon PBS seul.Aucun organelle cellulaire ne semble particulièrement visé par la toxine puisque les particules d'or étaient distribuées aléatoirement dans la cellule. Escherichia coli / entérotoxine STb / internalisation
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".