Characterization of Two<i>O</i>.-Methyltransferase-like Genes in Barley and Maize
Bibliographic record
Abstract
Tricin (3′,5′-dimethoxy-5,7,4′-trihydroxyflavone) is a characteristic flavone constituent of cereal grain plants that is credited for a variety of potential health benefits to humans. We have characterized the flavone-specific O.-methyltransferase genes of barley (Hordem vulgare. L., Gramineae), HvOMT1,. and maize (Zea mays. L., Gramineae), ZmOMT1., whose gene products use the flavone tricetin (5,7,3′,4′,5′-pentahydroxyflavone) as the preferred substrate and give rise to its 3′,5′-dimethyl derivative, tricin, as the major product. The fact that homologous enzymes catalyze the same reaction also in wheat and rice suggests the existence in cereal grain plants of a flavone-specific O.-methyltransferase multigene family. The natural occurrence of tricin in most monocot species implies the widespread occurrence of this gene family. The pharmacological significance of tricin as a naturally occurring constituent with a potential use as a nutraceutical, and the application of metabolic engineering methods to obtain tricin-enriched cereal grain products, are discussed.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".