Synthesis of disaccharide congeners of the <i>Trichinella spiralis</i> glycan and binding site mapping of two monoclonal antibodies
Bibliographic record
Abstract
The tetrasaccharide epitope, β-D-Tyvp(1[Formula: see text]3)β-D-GalNAcp(1[Formula: see text]4)[α-L-Fucp(1[Formula: see text]3)]β-D-GlcNAcp (1) is the major constituent of the N-glycan expressed on the cell surface of the parasite Trichinella spiralis. Two monoclonal antibodies (Mabs 9D4 and 18H1) that protect rats against infection by T. spiralis bind the terminal disaccharide epitope β-D-Tyvp(1[Formula: see text]3)β-D-GalNAcp conjugated to BSA. The syntheses of disaccharide congeners containing mono-deoxy, mono-methyl, as well as modifications to replace the acetamido group are reported. These target disaccharides were assayed for binding to the protective MAbs. For each antibody different clusters of three hydroxyl groups, that include C-2 and C-4 of tyvelose and for 18H1, the GalNAc acetamido group, provide the key polar interactions with the antibody binding sites. Mapping of the sites by functional group replacement revealed a similar pattern of recognition for the dideoxyhexose by the two MAbs while each recognizes distinct surfaces of the GalNAc residue. Consequently although both antibodies bury the 4-OH of tyvelose, the principal contact surface occurs on opposite sides of the 3,6-dideoxyhexose.Key words: β-tyveloside, 3,6-dideoxy-D-arabino-hexose, Trichinella carbohydrate antigen, antibody mapping, Trichinella spiralis, N-glycans, molecular recognition of carbohydrates, antigen topology, functional group replacement.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".