Rapid Acquirement of Transgenic Rice Plants Derived from Callus of Mature Embryos Transformed by Agrobacterium Mediation
Bibliographic record
Abstract
In this research we transformed calli of two elite germplasms, Taipei 309 and Nipponbare, derived from mature embryos using the construct of pCAMBIA0390 mediated by Agrobacterium tumefaciens strain EHA105. The expression plasmid pCAMBIA0390 was constructed with expressing elements of ubi:bar-Gus and ACT:Bt . Meanwhile We studied the effects of the factors on the regeneration rates of callus tissues. The results showed that the regeneration frequencies of Taipei 309 and Nipponbare increased up to 39% and 44% respectively, under the procedures as following: Callus was cultured 21days in the conditions of, 28℃, 2000 Lx, and 13 h/d illumination period. Infected callus air-dried 20 minutes and then co-cultured callus air-dried 20 minutes, finally, the callus desiccated using sterile filter paper of callus 8 hours. It shows that transformation cycle can shortened from 105 days to 60 days in absences of subculture stage. The GUS expression and PCR analysis indicated that the exogenous Bt gene has been integrated into the rice genome.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".