Characterization and varied expression of a membrane‐bound endo‐β‐1,4‐glucanase in hybrid poplar
Why this work is in the frame
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Bibliographic record
Abstract
To understand better the intricacies of secondary cell wall biosynthesis in trees, we investigated changes in cellulose chemistry and ultrastructure manifested by the mis-regulation of the poplar membrane-bound beta-1,4-endoglucanase orthologous to KORRIGAN (AtKOR). We isolated the poplar KORRIGAN gene from hybrid poplar (Populus albaxgrandidentata; designated PaxgKOR) and created a self-complementary (hairpin) RNAi suppression construct using PCR products derived from the gene. Additionally, AtKOR was employed to generate transgenic poplar over-expressing KORRIGAN. It was found that down-regulation leads to moderate to severe defects in plant growth, an irregular xylem (irx) phenotype, and significantly impacts the ultrastructure of the cellulose synthesized. The RNAi-suppressed lines deposited significantly reduced quantities of a more highly crystalline cellulose, while the hemicellulose content and, more specifically, the xylose content increased. In addition, the amount of soluble sucrose in the leaves and xylem decreased. Conversely, the AtKOR transgenics did not significantly alter cell wall development or plant growth parameters, but it did impact the ultrastructure of the cellulose produced, generating trees with less crystalline cellulose and reduced xylose content.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it