Improving Rhodamine B Fluorescence Thermometry in PDMS Microchannels by Photobleaching Absorbed Dye
Why this work is in the frame
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Bibliographic record
Abstract
A number of microfluidic applications require precise thermal control where in-channel temperature measurements are necessary during the prototyping stage. Rhodamine B based laser induced fluorescence is a common technique used to obtain high resolution measurement of the fluid temperature field. However, PDMS has a tendency to absorb small hydrophobic dyes, such as Rhodamine B, which results in a steady increase in the overall fluorescent signal. This increase in light intensity causes a significant problem that must be overcome to obtain reliable temperature measurements. In this work a simple technique is described to remove the fluorescent signal originating from absorbed Rhodamine B dye particles that does not require surface modification or any significant alterations to the experimental setup. Instead a high power light source is used to photobleach the particles prior to taking images for thermometry analysis. Herein we demonstrate the technique with a conventional fluorescence microscope and a 100W mercury arc lamp and study the temperature field at the intersection of a Y-channel PDMS/glass chip where hot and cold streams merge.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it