Target Binding Influences Permeability in Aptamer–Polyelectrolyte Microcapsules
Bibliographic record
Abstract
Aptamer-polyelectrolyte microcapsules are prepared for potential use as triggered delivery vehicles and microreactors. The hollow microcapsules are prepared from the sulforhodamine B aptamer and the polyelectrolytes poly(allylamine hydrochloride) and poly(sodium 4-styrene-sulfonate), using layer-by-layer (LbL) film deposition templated on a sacrificial CaCO(3) spherical core. Scanning electron microscopy and confocal microscopy confirm the formation of spherical CaCO(3) cores and LbL-aptamer microcapsules. Colocalization studies with fluorescently-tagged aptamer and sulforhodamine B verify the ability of the aptamer to recognize its cognate target in the presence of the K(+) ions that are required for its characteristic G-quadruplex formation. Fluorescence recovery after photobleaching studies confirms a significant difference in the permeability of the aptamer-polyelectrolyte microcapsules for the sulforhodamine B dye target compared to control microcapsules prepared with a random oligonucleotide. These results suggest that aptamer-based 'smart' responsive films and microcapsules could be applied to problems of catalysis and controlled release.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".