Milk Secretion and Ejection Are Impaired in the Mammary Gland of Mice Harboring a Cx43 Mutant While Expression and Localization of Tight and Adherens Junction Proteins Remain Unchanged1
Bibliographic record
Abstract
During pregnancy, the integrity of the mammary gland epithelium must be maintained by the junctional nexus formed by gap, adherens, and tight junctions for proper alveologenesis. We have previously shown that mammary glands from Gja1(Jrt/+) mice, harboring a mutation in the Gja1 gene encoding connexin (Cx) 43, have reduced Cx43 gap junction function and are unable to release milk to the pups. We hypothesized that low levels of Cx43 in the mammary gland may also affect other mammary gland connexins, tight and adherens junction proteins, and alveolar architecture, thus contributing to the lactation defect. Surprisingly, the levels and/or localization of Cx26 (Gjb2), Cx30 (Gjb6), Cx32 (Gjb1), E-cadherin, P-cadherin, beta-catenin, claudin-1, and occludin were all unaffected by the decreased levels of Cx43 at parturition, suggesting no cross-talk to other connexins or junctional proteins in mutant mice mammary glands. Consistently, in mammary gland myoepithelial primary cultures or in Normal Rat Kidney (NRK) cells, tight and adherens junction protein levels and localization were unaffected by the expression of Cx43 mutants. However, whereas the architecture of the alveolar and ductal structures appeared to be normal in mutant mice, the presence of milk and cytoplasmic lipid droplets were frequently observed within the epithelium itself, suggesting a defect in the secretory process. Overall, these results suggest that proteins associated with tight and adherens junctions are unaffected by greatly reduced Cx43 gap junctions in the mammary gland epithelium, and they further suggest that the mammary gland defect in Cx43 mutant mice may extend to impaired milk secretion.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".