Worldwide Epidemiology of Neutralizing Antibodies to Adeno‐Associated Viruses
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
Abstract
Recombinant adeno-associated viruses (AAVs) have unique gene-transfer properties that speak to their potential as carriers for gene therapy or vaccine applications. However, the presence of neutralizing antibodies to AAV as a result of previous exposure can significantly limit effective gene transfer. In this study, we obtained 888 human serum samples from healthy volunteers in 10 countries around the world. Samples were assayed for neutralizing antibodies to AAV1, AAV2, AAV7, and AAV8, as well as to a novel, structurally distinct AAV vector, rh32.33, in an in vitro transduction inhibition assay. Our data revealed that neutralizing antibodies to AAV2 were the most prevalent antibodies in all regions, followed by antibodies to AAV1. The seroprevalences of antibodies to AAV7 and to AAV8 were lower than that for antibodies to AAV1, and neutralization of AAVrh32.33 was only rarely detected. Our data also indicate a strong linkage of seroreactivity between apparently distinct serotypes that has not been predicted previously in animal models.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- The Journal of Infectious Diseases
- Topic
- Virus-based gene therapy research
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- —
- Funders
- Institute of GeneticsNational Institute of Diabetes and Digestive and Kidney DiseasesNational Heart, Lung, and Blood InstituteVlaamse regeringUniversity of PennsylvaniaGlaxoSmithKlineNational Institutes of HealthInternational AIDS Vaccine Initiative
- Keywords
- VirologyAntibodyAdeno-associated virusNeutralizationNeutralizing antibodyTransduction (biophysics)BiologySerotypeRecombinant DNAVirusImmunologyGeneGeneticsVector (molecular biology)
- Has abstract in OpenAlex
- yes