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Large-Scale Protein-Protein Interaction Detection Approaches: Past, Present and Future

2008· article· en· W2172097289 on OpenAlex
N. Chepelev, Leonid Chepelev, Mahiuddin Alamgir, Ashkan Golshani

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueBiotechnology & Biotechnological Equipment · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBioinformatics and Genomic Networks
Canadian institutionsCarleton University
Fundersnot available
KeywordsProtein–protein interactionProtein Interaction NetworksComputational biologyTandem affinity purificationField (mathematics)Computer scienceInteraction networkScale (ratio)Data scienceBiologyGeneticsGeneBiochemistryAffinity chromatography

Abstract

fetched live from OpenAlex

Protein-protein interaction elucidation is of immense importance to biology, medicine, and related fields. It is now realized that various diseases such as different types of cancers, Alzheimer's disease, etc, require an integrated view of protein interaction networks. To aid in deciphering these networks, a number of methods have been developed including yeast two-hybrid analysis, tandem affinity purification tagging, as well as protein microarray technologies. In this article, we discuss some of the most important trends and technologies of the past, reflect on their present, and explore some exciting future directions in the field of large-scale protein-protein interaction detection. We argue that the future of the protein interaction elucidation field lies in the development of novel and/or improved high-throughput techniques that generate reproducible and most importantly, quantitative data.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.376
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0020.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.218
Teacher spread0.201 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it