Genetic Polymorphisms of Mitochondrial Genome D-loop Region in Bakhtiarian Population by PCR-RFLP
Bibliographic record
Abstract
The human mitochondrial DNA is a 16569 bp closed, circular molecule that was sequenced and mapped. The D-loop of mitochondrial DNA is a major control site for mtDNA expression and it is important in maternal inheritance. This region in different population is vary and contains essential transcription and replication elements and it could be used for detection of mother inheritance, and human evolution. The aim of present study was to determine the D-loop region polymorphism in Bakhtiarian population in southwest Iran. The blood samples of 168 healthy people of Bakhtiarian population in Iran were collected and total DNA was extracted and then, mtDNA D-loop region was amplified by PCR using specific primers. Restriction fragment length polymorphism (RFLP) was analyzed in PCR products by HaeIII and AluI restriction endonuclease. Amplified fragments for mtDNA D-loop region on 1% agarose gel revealed a fragment of about 1024 bp. The results of this study showed 5 restriction patterns for HaeIII enzyme (with 1 heteroplasmy) and 2 restriction patterns for AluI enzyme (with 2 heteroplasmies) in Bakhtiarian population. The findings showed a low level of genetic polymorphism in D-loop region and it is related to high kinship marriages and low range of migration in Bakhtiarian population. Keywords: mtDNA, D-loop region, RFLP, Polymorphism, Bakhtiarin population, Iran
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".