Intracellular Ice Formation in Three-Dimensional Tissues: Pancreatic Islets
Bibliographic record
Abstract
The formation of ice within living cells during freezing is typically a lethal event. Design of optimal cryopreservation protocols therefore requires the ability to define the conditions under which intracellular ice formation (IIF) will occur. A model for IIF in cells was adapted to predict the probability of IIF in the multicellular pancreas islet during freezing, and experimental data were obtained via cryomicroscopy for a variety of freezing conditions. The influence of a cryoprotective agent, dimethyl sulfoxide (Me2SO), on IIF was evaluated. The model was applied to evaluate the sensitivity of IIF to a number of key physiological and protocol parameters. Agreement between the model and experimental data for IIF in pancreas islets in 2 M Me2SO is quite good for cooling rates of 2°C/min and higher and temperatures to - 50°C.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".