Construction and verification of double T-DNA plant expression vector of the DREB gene
Bibliographic record
Abstract
The W34 gene, which was first cloned into pGEM-T-EASY vector,was inserted into EcoRI site of pBDREB vector.A plasmid was selected, with which the bacteria host could grow on Amp plate; The plasmids pBW34-DREB、pSPROK、pBlueks were digested with the restriction enzymes PstI/KpnI、KpnI/EcoRI、PstI/EcoRI respectively, so three fragments were obtained, and then they were ligated with T4 DNA Ligase, thus we got the medium expression vector pBWD-Tnos;The vector pBWD-Tnos was cut with SmaI and EcoRV, a 2.16kb fracment including promotor and coding sequence of DREB was obtained.The fragment was then ligated to the vector pCDMAR-Hyg which had been cut with an enzyme SmaI, so the plant expression vector pCDMAR-pWDT-Hyg of the DREB protein encoding gene was finally obtained.The results of the restriction enzymes digestion demonstrated the correctness of the construction. The double T-DNA vector was transferred into the maize embryonic calli mediated by Agrobacterium tumefaciens. We hope we can obtain the transgene plants without selective marker genes.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".