Comparison Between Animal Infectivity and Nucleic Acid Staining for Determination of Viability of Ozone-Inactivated<i>Cryptosporidium parvum Oocysts</i>
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Abstract The viability of untreated and ozone treated Cryptosporidium parvum oocysts was determined using the nucleic acid dye SYTOO® staining evaluated by epi-fluorescence microscopy and flow cytometry. The use of microscopy to enumerate ozone treated oocysts, stained with SYTO-9, was found to either over- or underestimate the reduction in infectivity of parasites depending on the level of ozonation. Flow cytometry fluorescence histograms were generated for oocysts exposed to a range of ozone contact times. The median fluorescence intensity of ozone treated oocysts was found to be proportional to the reduction in mouse infectivity. A general linear regression model was fitted to the SYTO-9 staining data to give a predictive model for use with flow cytometry which provides a good estimate of the reduction in infectivity of C. parvum oocysts exposed to ozone in laboratory water. Key Words: OzoneDisinfectionCryptosporidium parvumViability AssayNucleic Acid Dye; SYTO-9Epi-fluorescence MicroscopyFlow CytometryAnimal InfectivityLogit-responseIncomplete Gamma Horn Additional informationNotes on contributorsMiodrag Belosevic Professor, CW-405 Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, CANADA Telephone: (780) 492-1266; Fax: (780) 492-9234;E-mail: mike.belosevicffljualberta.ca
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it