Development of SCAR Marker Linked to Sex Determination Locus in<i> Trichosanthes dioica</i>
Why this work is in the frame
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Bibliographic record
Abstract
Pointed gourd ( Trichosanthes dioica Roxb .), a perennial herb of family Cucurbitaceae, is morphologically different from the other cucurbits due to its dioecism and vegetative means of propagation. Breeding in pointed gourd is less viable due to poor germination, vegetative propagation mean and dioecy. A reliable method for determining the gender of pointed gourd at seedling stage would facilitate breeding programs. With this aim, RAPD markers were used to identify a sex linked marker. RAPD profiles with 104 primers were analyzed in 10 male and 44 female plants for presence/absence of specific bands. Two sex-specific fragments, OPC05 1000 and OPK07 300 , associated with maleness and femaleness, respectively, were converted in SCAR markers. Based on the sequence of OPC5 1000 and OPK-07 300 , five and one pair of specific forward and reverse SCAR markers were designed, respectively. Among five male specific SCAR, only one marker (SCF/R-3 628 ) discriminated both sexes. Thus, SCAR designed from RAPD fragment could be used as an effective and reliable marker for early sex diagnosis and to reduce both time and costs associated with breeding in T. dioeca .
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it