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Record W2181067433 · doi:10.1002/cyto.a.22732

A benchmark for evaluation of algorithms for identification of cellular correlates of clinical outcomes

2015· article· en· W2181067433 on OpenAlexafffund
Nima Aghaeepour, Pratip K. Chattopadhyay, Maria Chikina, Tom Dhaene, Sofie Van Gassen, Miron B. Kursa, Bart N. Lambrecht, Mehrnoush Malek, Geoffrey J. McLachlan, Qian Yu, Peng Qiu, Yvan Saeys, Rick Stanton, Dong Tong, Celine Vens, Sławomir Walkowiak, Kui Wang, Greg Finak, Raphaël Gottardo, Tim R. Mosmann, Garry P. Nolan, Richard H. Scheuermann, Ryan R. Brinkman

Bibliographic record

VenueCytometry Part A · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsUniversity of British ColumbiaBC Cancer Agency
FundersNational Institute of Allergy and Infectious DiseasesNational Institute of Biomedical Imaging and BioengineeringVlaamse regeringU.S. Public Health ServiceNational Cancer InstituteOvarian Cancer Research FundNational Institutes of HealthNatural Sciences and Engineering Research Council of CanadaLupus Research AllianceInternational Society for Advancement of CytometryCanada's Michael Smith Genome Sciences CentreNational Heart, Lung, and Blood InstituteU.S. Department of Defense
KeywordsFlow cytometryPeripheral blood mononuclear cellAlgorithmCytometryPopulationBenchmark (surveying)Identification (biology)Computer scienceMedicineImmunologyBiologyIn vitro

Abstract

fetched live from OpenAlex

The Flow Cytometry: Critical Assessment of Population Identification Methods (FlowCAP) challenges were established to compare the performance of computational methods for identifying cell populations in multidimensional flow cytometry data. Here we report the results of FlowCAP-IV where algorithms from seven different research groups predicted the time to progression to AIDS among a cohort of 384 HIV+ subjects, using antigen-stimulated peripheral blood mononuclear cell (PBMC) samples analyzed with a 14-color staining panel. Two approaches (FlowReMi.1 and flowDensity-flowType-RchyOptimyx) provided statistically significant predictive value in the blinded test set. Manual validation of submitted results indicated that unbiased analysis of single cell phenotypes could reveal unexpected cell types that correlated with outcomes of interest in high dimensional flow cytometry datasets.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.023
metaresearch head score (Gemma)0.064
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: none
Teacher disagreement score0.023
Threshold uncertainty score0.123

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0230.064
Meta-epidemiology (narrow)0.0020.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0030.002
Science and technology studies0.0010.001
Scholarly communication0.0020.001
Open science0.0020.002
Research integrity0.0030.002
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.149
GPT teacher head0.402
Teacher spread0.253 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations75
Published2015
Admission routes2
Has abstractyes

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Same venueCytometry Part ASame topicSingle-cell and spatial transcriptomicsFrench-language works237,207